Smad4、Smad7和Caveolin-1在Wistar大鼠口腔黏膜癌变中的表达和意义

doi:10.19439/j.sjos.2017.04.012

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (4): 409-413.doi: 10.19439/j.sjos.2017.04.012

Smad4、Smad7和Caveolin-1在Wistar大鼠口腔黏膜癌变中的表达和意义

李媛¹, 周建炜², 李慧良¹, 刘进忠³

1.郑州市第一人民医院口腔科,河南郑州 450004；
2.郑州大学人民医院·河南省人民医院肿瘤内科,河南郑州 450003；
3.郑州大学口腔医学院,河南郑州 450052

收稿日期:2016-10-21 修回日期:2017-02-10 出版日期:2017-08-25 发布日期:2017-09-01
通讯作者: 刘进忠,E-mail:drliujz@126.com
作者简介:李媛（1978-）,女,硕士,主治医师,E-mail: zzuly@163.com

Significance of Smad4, Smad7 and Caveolin-1 expression in oral mucosa carcinogenesis of Wistar rats

LI Yuan¹, ZHOU Jian-wei², LI Hui-liang¹, LIU Jin-zhong³

1.Department of Stomatology, Zhengzhou First People's Hospital. Zhengzhou 450004;
2.Department of Oncology, People's Hospital of Zhengzhou University. Henan Provincial People's Hospital. Zhengzhou 450003;
3.School of Stomatology, Zhengzhou University. Zhengzhou 450052, Henan Province, China

Received:2016-10-21 Revised:2017-02-10 Online:2017-08-25 Published:2017-09-01

摘要/Abstract

摘要： 目的研究母亲DPP同源物4 (mothers against decapentaplegic homolog 4,Smad4)、母亲DPP同源物7 (mothers against decapentaplegic homolog 7,Smad7)和小窝蛋白1 (Caveolin-1)在Wistar大鼠口腔黏膜癌变过程中的表达,了解TGF-β/Smad信号传导通路和小窝蛋白Caveolin-1在口腔癌前病变中的作用。方法采用郑州大学口腔医学院存档的Wistar大鼠口腔腭部黏膜癌变标本,包括正常黏膜5例,单纯增生黏膜10例,轻度异常增生黏膜6例,中度异常增生黏膜7例,重度异常增生黏膜13例,口腔癌变组织28例。应用免疫组织化学SP法检测Smad4、Smad7和Caveolin-1蛋白的表达,采用SPSS15.0软件包对数据进行统计学分析。结果Smad4蛋白在正常和单纯增生黏膜、上皮异常增生黏膜和口腔癌黏膜中表达依次降低,3组间差异显著（P<0.05）;Smad7和Caveolin-1蛋白在正常和单纯增生黏膜、上皮异常增生黏膜和口腔癌黏膜中表达均依次升高,3组间差异显著(P<0.05)。经Spearman相关分析,Smad4与Smad7蛋白表达呈负相关,Smad4与Caveolin-1蛋白表达呈负相关,Smad7与Caveolin-1蛋白表达呈正相关（P<0.05）。结论Smad4、Smad7和Caveolin-1蛋白在口腔黏膜癌变过程中可能存在协同作用。

关键词: 口腔癌, 上皮异常增生, Smad4, Smad7, Caveolin-1

Abstract: To investigate the expression of Smad4, Smad7 and Caveolin-1 in the process of carcinogenesis of oral mucosa in Wistar rats, and to understand the changes of TGF-β/Smad signaling pathway and Caveolin-1 in oral cancer. METHODS: Palatal mucosal carcinogenesis specimens of Wistar rats were obtained from School of Stomatology, Zhengzhou University, which included 5 samples of normal mucosa, 10 samples of simple hyperplasia mucosa, 6 samples of mild mucosal dysplasia, 7 samples of moderate mucosal dysplasia, 13 samples of mucosa severe mucosal dysplasia, and 28 samples of oral cancer tissue. The expression of Smad4, Smad7 and Caveolin-1 was detected by immunohistochemistry. SPSS15.0 software package was used for statistical analysis. RESULTS: The expression of Smad4 decreased in normal and hyperplastic epithelia, dysplasticepithelia and oral cancer gradually, the difference of the expression among the three groups was significant (P<0.05). The expression of Smad7 and Caveolin-1 increased in normal and hyperplastic epithelia, dysplasticepithelia and oral cancer gradually, respectively; the difference of the expression among the 3 groups was significant (P<0.05). Spearman correlation analysis showed that Smad4 was negatively correlated with Smad7, Smad4 was negatively correlated with caveolin-1, Smad7 was positively correlated with Caveolin-1 (P<0.05). CONCLUSIONS: Synergistic effects may exist among Smad4, Smad7 and caveolin-1 in carcinogenesis of oral cancer.

Key words: Oral cancer, Dysplasticepithelia, Smad4, Smad7, Caveolin-1

中图分类号:

R781.5

李媛, 周建炜, 李慧良, 刘进忠. Smad4、Smad7和Caveolin-1在Wistar大鼠口腔黏膜癌变中的表达和意义[J]. 上海口腔医学, 2017, 26(4): 409-413.

LI Yuan, ZHOU Jian-wei, LI Hui-liang, LIU Jin-zhong. Significance of Smad4, Smad7 and Caveolin-1 expression in oral mucosa carcinogenesis of Wistar rats[J]. Shanghai Journal of Stomatology, 2017, 26(4): 409-413.

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Smad4、Smad7和Caveolin-1在Wistar大鼠口腔黏膜癌变中的表达和意义

Significance of Smad4, Smad7 and Caveolin-1 expression in oral mucosa carcinogenesis of Wistar rats

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