SOCS-1和SOCS-3在慢性根尖周炎病损组织中的表达及临床意义

doi:10.19439/j.sjos.2017.04.007

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (4): 384-388.doi: 10.19439/j.sjos.2017.04.007

SOCS-1和SOCS-3在慢性根尖周炎病损组织中的表达及临床意义

王丝墨, 马楠, 仇丽鸿, 李晓琳, 杨谛, 薛明

中国医科大学口腔医学院牙体牙髓病科,辽宁省口腔医学研究所牙体牙髓病学研究室,辽宁沈阳 110002

收稿日期:2016-12-19 修回日期:2017-03-31 出版日期:2017-08-25 发布日期:2017-09-01
通讯作者: 仇丽鸿,E-mail:drqlh@yahoo.com
作者简介:王丝墨（1991-）,女,硕士,医师,E-mail:wangsimo2792@126.com
基金资助:
国家自然科学基金（2015-A-0000000081500843）; 沈阳市科技计划项目（F15-199-1-56）

Expression of SOCS-1 and SOCS-3 in chronic periapical lesions and its clinical significance

WANG Si-mo, MA Nan, QIU Li-hong, LI Xiao-lin, YANG Di, XUE Ming

Department of Endodontics, School of Stomatology, China Medical University; Lab of Endodontic, Liaoning Institute of Dental Research. Shenyang 110002, Liaoning Province, China

Received:2016-12-19 Revised:2017-03-31 Online:2017-08-25 Published:2017-09-01

摘要/Abstract

摘要： 目的检测细胞因子信号转导抑制因子1（suppressor of cytokine signaling-1,SOCS-1）和细胞因子信号转导抑制因子3（suppressor of cytokine signaling-3,SOCS-3）在慢性根尖周炎病损组织中的表达水平,探讨SOCS-1、3在慢性根尖周炎发病机制中的作用。方法收集25例慢性根尖周炎病损组织作为病例组,16例健康牙的牙周膜组织作为对照组。对样本中的蛋白表达量采用免疫组织化学法检测,mRNA表达量采用实时定量PCR法检测。采用SPSS13.0软件包对数据进行统计学分析。结果免疫组织化学结果显示,SOCS-1、3 蛋白在慢性根尖周炎病损组织中的表达水平均显著高于对照组（P<0.01）;重度炎症组中,SOCS-1、3 的蛋白表达显著高于轻中度炎症组（P<0.01）。SOCS-1 mRNA在轻中度炎症组、重度炎症组和对照组中的表达量分别为2.620±1.552、2.373±1.083和1.277±1.040,SOCS-3 mRNA的表达量分别为9.308±5.901,7.565±3.233和1.232±1.099。SOCS-1、3 mRNA在轻中度炎症组、重度炎症组均显著高于对照组（P<0.01）,但不同炎症组之间并无显著差异。结论SOCS-1、3可能与慢性根尖周炎密切相关。

关键词: 慢性根尖周炎, 细胞因子信号转导抑制因子1, 细胞因子信号转导抑制因子3, 实时荧光定量PCR

Abstract: To detect the expression of suppressor of cytokine signaling-1 (SOCS-1) and suppressor of cytokine signaling-3 (SOCS-3) in chronic periapical lesions and to clarify their roles in pathogenesis of apical periodontitis. METHODS: A total of 25 chronic periapical lesion tissues and 16 normal periodontal ligament tissues were collected respectively. The expression of mRNA was measured by real-time PCR, the protein expression was measured by immunohistochemical analysis. Statistical analysis was performed using SPSS 13.0 software package. RESULTS: Immunohistochemical results indicated that expression levels of SOCS-1 and SOCS-3 protein in chronic periapical lesions were significantly higher than that in normal tissues (P<0.01); Moreover, SOCS-1 and SOCS-3 protein expression levels in severe inflammation group were significantly higher than that in mild inflammation group (P<0.01). In mild inflammation group, severe inflammation group and control group, the expression levels of SOCS-1 mRNA were 2.620±1.552, 2.373±1.083 and 1.277±1.040, whereas those of SOCS-3 were 9.308±5.901, 7.565±3.233 and 1.232±1.099, respectively. CONCLUSIONS: The expression levels of SOCS-1 mRNA and SOCS-3 mRNA are significantly higher both in mild inflammation group and severe inflammation group than that in control group (P<0.01), while no significant difference is found in mild and severe inflammation group.

Key words: SOCS-1, SOCS-3, Real-time PCR, Chronic apical periodontitis

中图分类号:

R781.3

王丝墨, 马楠, 仇丽鸿, 李晓琳, 杨谛, 薛明. SOCS-1和SOCS-3在慢性根尖周炎病损组织中的表达及临床意义[J]. 上海口腔医学, 2017, 26(4): 384-388.

WANG Si-mo, MA Nan, QIU Li-hong, LI Xiao-lin, YANG Di, XUE Ming. Expression of SOCS-1 and SOCS-3 in chronic periapical lesions and its clinical significance[J]. Shanghai Journal of Stomatology, 2017, 26(4): 384-388.

参考文献

[1] 樊明文. 牙体牙髓病学[M]. 第4版. 北京: 人民卫生出版社, 2012: 227.
[2] Stashenko P, Teles R, D'Souza R. Periapical inflammatory responses and their modulation [J]. Crit Rev Oral Biol Med, 1998, 9(4): 498-521.
[3] 马楠, 曲柳, 徐莉雅, 等. IL-34 在慢性根尖周病损组织中的表达及临床意义 [J]. 上海口腔医学, 2016, 25(1): 53-57.
[4] Linossi EM, Nicholson SE. Kinase inhibition, competitive binding and proteasomal degradation: resolving the molecular function of the suppressor of cytokine signaling (SOCS) proteins [J]. Immunol Rev, 2015, 266(1): 123-133.
[5] Starr R, Willson TA, Viney EM, et al. A family of cytokine-inducible inhibitors of signalling [J]. Nature, 1997, 387(6636): 917-921.
[6] Endo TA, Masuhara M, Yokouchi M, et al. A new protein containing an SH2 domain that inhibits JAK kinases [J]. Nature, 1997, 387(6636): 921-924.
[7] Gao A, Van Dyke TE. Role of suppressors of cytokine signaling 3 in bone inflammatory responses[J]. Front Immunol, 2013, 4: e506.
[8] Papathanasiou E, Kantarci A, Konstantinidis A, et al. SOCS-3 regulates alveolar bone loss in experimental periodontitis [J].J Dent Res, 2016, 95(9): 1018-1025.
[9] 孙晓辉. 细胞因子信号抑制因子在骨关节炎患者软骨细胞的表达 [J]. 细胞与分子免疫学杂志, 2013, 29(2): 186-189.
[10] 徐莉雅, 马楠, 仇丽鸿, 等. Wnt5a在慢性根尖周炎病损中的表达及临床意义 [J]. 上海口腔医学, 2016, 24(4): 470-474
[11] 胡跃云, 吴宏菊, 辛彦, 等. HuR蛋白在胃癌组织中的表达及其意义 [J]. 中华临床医师杂志(电子版), 2011, 5(1): 91-95.
[12] de Souza JA, Nogueira AV, de Souza PP, et al. Expression of suppressor of cytokine signaling 1 and 3 in ligature-induced periodontitis in rats[J]. Arch Oral Biol, 2011, 56(10): 1120-1128.
[13] Menezes R, Garlet TP, Trombone AP, et al. The potential role of suppressors of cytokine signaling in the attenuation of inflammatory reaction and alveolar bone loss associated with apical periodontitis [J]. J Endod, 2008, 34(12): 1480-1484.
[14] Garlet GP, Cardoso CR, Campanelli AP, et al. Expression of suppressors of cytokine signaling in diseased periodontal tissues: a stop signal for disease progression?[J]. J Periodontal Res, 2006, 41(6): 580-584.
[15] Chaves de Souza JA, Nogueira AV, Chaves de Souza PP, et al. SOCS3 expression correlates with severity of inflammation, expression of proinflammatory cytokines, and activation of STAT3 and p38 MAPK in LPS-induced inflammation in vivo [J]. Mediators Inflamm, 2013, 2013: 650812.
[16] 李任, 仇丽鸿. 牙髓卟啉单胞菌内毒素致病性研究进展 [J]. 中国实用口腔科杂志, 2009, 2(12): 756-758.
[17] Kimura A, Naka T, Muta T, et al. Suppressor of cytokine signaling-1 selectively inhibits LPS-induced production by regulating JAK-STAT[J]. Proc Natl Acad Sci USA, 2005, 102(47): 17089-17094.
[18] Yasukawa H, Ohishi M, Mori H, et al. IL-6 induces an anti-inflammatory response in the absence of SOCS3 in macrophages [J]. Nat Immunol, 2003, 4(6): 551-556.
[19] Fukushima A, Kajiya H, Izumi T, et al. Pro-inflammatory cytokines induce suppressor of cytokine signaling-3 in human periodontal ligament cells[J]. J Endod, 2010, 36(12): 1004-1008.

SOCS-1和SOCS-3在慢性根尖周炎病损组织中的表达及临床意义

Expression of SOCS-1 and SOCS-3 in chronic periapical lesions and its clinical significance

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