七氟烷对血管内皮细胞迁移能力的影响

doi:10.19439/j.sjos.2017.03.008

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (3): 272-276.doi: 10.19439/j.sjos.2017.03.008

七氟烷对血管内皮细胞迁移能力的影响

孙元青, 梁冰, 张磊, 徐辉

上海交通大学医学院附属第九人民医院麻醉科,上海 200001

收稿日期:2016-11-15 修回日期:2017-01-20 出版日期:2017-06-25 发布日期:2017-07-05
通讯作者: 徐辉,E-mail：1268dh@163.com
作者简介:孙元青（1992-）,女,硕士,E-mail：15000058831@163.com

Effects of sevoflurane on the migration of vascular endothelial cells

SUN Yuan-qing, LIANG Bing, ZHANG Lei, XU Hui

Department of Anesthesiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine. Shanghai 200011, China

Received:2016-11-15 Revised:2017-01-20 Online:2017-06-25 Published:2017-07-05

摘要/Abstract

摘要： 目的探讨七氟烷对血管内皮细胞迁移功能的影响及其分子生物学机制。方法将人脐静脉内皮细胞（human umbilical vein endothelial cells,HUVECs）随机分为4组（每组包含4个直径为10 cm的培养皿）,对照组、七氟烷暴露组0.5 h组、1 h组和2 h组。对照组为正常环境培养的细胞,七氟烷暴露组培养环境包括5% CO₂、21% O₂和2%七氟烷（相当于1.6个MAC）;对4组细胞分别进行划痕实验,观察HUVECs的迁移能力,并对各组HUVECs采用RT-PCR检测血管内皮细胞钙黏蛋白 mRNA（VE-cadherin mRNA）的表达情况。采用SPSS13.0软件包对数据进行统计学分析。结果划痕实验12 h后,与对照组相比,在2%七氟烷中暴露2 h的HUVECs迁移距离显著缩短（P<0.01）,细胞迁移功能受到显著抑制,而在2%七氟烷中暴露0.5 h和1 h的HUVECs迁移距离与对照组相比均无显著差异。RT-PCR实验结果显示,在2%七氟烷中暴露2 h的HUVECs与对照组相比, VE-cadherin mRNA 表达显著上调（P<0.05）。结论2%七氟烷暴露2 h对HUVECs迁移功能具有显著抑制作用,其机制可能与七氟烷上调VE-cadherin的表达有关。

关键词: 七氟烷, 迁移功能, 人脐静脉内皮细胞, 血管内皮钙黏蛋白

Abstract: PURPOSE: To evaluate the effects of sevoflurane on cell migration and expression of VE-cadherin mRNA in human umbilical vein endothelial cells(HUVECs). METHODS: HUVECs were allocated to 4 groups randomly (each group contained 4 culture dishes with a diameter of 10 cm), and were exposed to 2% sevoflurane (refer to 1.6 MAC) respectively for 0 h (control group which exposed to the same environment as the other 3 groups but without sevoflurane), 0.5 h, 1 h, 2 h. After sevoflurane exposure, HUVECs were subjected to scratch assay to observe the migration distance; RT-PCR was used to detect the expression of vascular endothelial-cadherin mRNA(VE-cadherin mRNA). Statistical analysis of the data was conducted using SPSS 13.0 software package. RESULTS: Scratch assay results suggested that the migration ability of HUVECs exposed to 2% sevoflurane for 2 h were significantly inhibited during 12 h after scratch compared with the control group(P<0.01), and RT-PCR results suggested that HUVECs exposed to 2% sevoflurane for 2 h expressed greater VE-cadherin mRNA than the control group(P<0.05). CONCLUSIONS: Sevoflurane exposure for 2 h can inhibit HUVECs migration and the mechanism may be up-regulating VE-cadherin expression.

Key words: Sevoflurane, Migration, HUVECs, VE-cadherin

中图分类号:

R614.2

孙元青, 梁冰, 张磊, 徐辉. 七氟烷对血管内皮细胞迁移能力的影响[J]. 上海口腔医学, 2017, 26(3): 272-276.

SUN Yuan-qing, LIANG Bing, ZHANG Lei, XU Hui. Effects of sevoflurane on the migration of vascular endothelial cells[J]. Shanghai Journal of Stomatology, 2017, 26(3): 272-276.

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七氟烷对血管内皮细胞迁移能力的影响

Effects of sevoflurane on the migration of vascular endothelial cells

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