人下颌骨和髂骨来源间充质细胞的分子表达差异

doi:10.19439/j.sjos.2017.02.011

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (2): 180-183.doi: 10.19439/j.sjos.2017.02.011

人下颌骨和髂骨来源间充质细胞的分子表达差异

杨荃荃¹, 王娇¹, 李志勇²

1.南充市中心医院口腔科,2.耳鼻喉科,四川南充 637900

收稿日期:2016-09-12 修回日期:2016-11-12 出版日期:2017-04-25 发布日期:2017-05-04
通讯作者: 李志勇,E-mail: zhiyong_li16@tom.com
作者简介:杨荃荃（1979-）,女,硕士研究生,副主任医师,E-mail: yangquanquandental@163.com

Differentially expressed genes between the human mandible- and ilium- derived mesenchymal cells

YANG Quan-quan¹, WANG Jiao¹, LI Zhi-yong²

1.Department of Stomatology, 2.Department of Otolaryngology, Nanchong Central Hospital. Nanchong 637900, Sichuan Province, China

Received:2016-09-12 Revised:2016-11-12 Online:2017-04-25 Published:2017-05-04

摘要/Abstract

摘要： 目的: 比较人下颌骨和髂骨来源细胞的分子表达差异。方法: 从GEO公共数据库中,通过GEO2R软件分析获得在人下颌骨和髂骨来源细胞中差异表达的基因。应用DAVID和Gene MANIA在线数据库对这些基因之间的关系进行生物信息学分析。结果: 从GEO数据获得了在人下颌骨和髂骨来源细胞中差异表达明显的293个基因,这些基因主要是同源盒基因,与骨骼发育密切相关。这些基因经DAVID数据库分析后,被富集到“skeletal system development”、“forebrain development”、“limb development”、“WNT信号通路”等不同的子集。经Gene MANIA在线数据库分析后,建立了MSX1、MSX2、HAND2、SIX1、PITX2、OSR2、PAX3和PRRX1等8个在人下颌骨与髂骨来源间充质细胞中表达差异明显的基因分子网络,这些基因通过共表达、共定位、遗传学相互作用等彼此关联。结论: 下颌骨和髂骨来源间充质细胞存在差异化的分子调控,提示在应用髂骨细胞作为种子细胞对颌骨进行组织工程修复时,可考虑通过对髂骨间充质细胞进行基因修饰,以使其更接近颌骨间充质细胞特性。

关键词: 下颌骨, 髂骨, 间充质细胞, 分子表达差异

Abstract: PURPOSES: To explore the differentially expressed genes between human mandible- and ilium- derived mesenchymal cells. METHODS: Differentially expressed genes between the human mandible- and ilium- derived mesenchymal cells were obtained from the GEO database based on the analysis of GEO2R software, then the relationship among these genes were analysed using DAVID and Gene MANIA on-line database. RESULTS: Two hundred and ninety three differentially expressed genes between the human mandible and iliac derived mesenchymal cells were obtained, and most of them were homeobox genes that related to bone development. These genes could also be enriched into different subgroups based on the analysis of DAVID database, such as "skeletal system development", "forebrain development", "limb development" and "WNT signaling pathway". After the analysis from Gene MANIA database, the molecular networks of MSX1, MSX2, HAND2, SIX1, PITX2, OSR2, PAX3 and PRRX1 were built to show that these eight genes exhibited interactions, including co-expression, co-localization, genetic interactions. CONCLUSIONS: There are different gene regulating mechanisms between the human mandible- and ilium- derived mesenchymal cells. It implies that genetic modification may be applied to get iliac derived mesenchymal cells to be more suitable for tissue-engineered repair of jaw.

Key words: Mandible, Ilium, Mesenchymal cells, Differentially expressed genes

中图分类号:

R782.1

杨荃荃, 王娇, 李志勇. 人下颌骨和髂骨来源间充质细胞的分子表达差异[J]. 上海口腔医学, 2017, 26(2): 180-183.

YANG Quan-quan, WANG Jiao, LI Zhi-yong. Differentially expressed genes between the human mandible- and ilium- derived mesenchymal cells[J]. Shanghai Journal of Stomatology, 2017, 26(2): 180-183.

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人下颌骨和髂骨来源间充质细胞的分子表达差异

Differentially expressed genes between the human mandible- and ilium- derived mesenchymal cells

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