氟对大鼠成釉细胞GRP-78和caspase-12表达的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (1): 1-5.

• 基础研究 • 下一篇

氟对大鼠成釉细胞GRP-78和caspase-12表达的影响

李健,张颖,马林,张凯强,雷双,顾何锋

中国医科大学附属口腔医院口腔预防科,辽宁省口腔医学研究所口腔预防医学教研室,中国医科大学附属口腔医院中心实验室,辽宁沈阳 110002

收稿日期:2014-03-14 出版日期:2015-02-20 发布日期:2015-07-24
通讯作者: 张颖,Tel:024-22895293,E-mail:zhangyingcmu@126.com
作者简介:李健（1988-）,在读硕士研究生,E-mail:794239430@qq.com
基金资助:
国家自然科学基金（81072245）; 辽宁省自然科学基金（20102278）

The effect of fluoride on the expression of GRP-78 and caspase-12 in rat ameloblast

LI Jian,ZHANG Ying,MA Lin,ZHANG Kai-qiang,LEI Shuang,GU He-feng

Department of Preventive Dentistry, Central Laboratory, School of Stomatology, China Medical University. Shenyang 110002, Liaoning Province, China

Received:2014-03-14 Online:2015-02-20 Published:2015-07-24
Supported by:
National Natural Science Foundation of China (81072245) and Natural Science Foundation of Liaoning Province (20102278)

摘要/Abstract

摘要： 目的研究GRP-78和caspase-12在氟致大鼠成釉细胞内质网应激和细胞凋亡中的作用,探讨氟所介导的内质网应激是否导致细胞凋亡。方法应用CCK8和流式细胞术观察不同浓度的氟对成釉细胞活性及凋亡数量的影响,以实时定量PCR和Western印迹法检测氟对内质网伴侣分子GRP-78和caspase-12基因和蛋白表达的影响,应用SPSS13.0软件包对数据进行统计学分析。结果随着氟浓度的不断增加,大鼠成釉细胞活性呈逐渐下降趋势,流式细胞术同样显示发生凋亡的细胞数量逐渐上升;实时定量RT-PCR和Western印迹结果显示,GRP-78和caspase-12的表达量随着氟浓度增加而增加。结论过量氟介导了大鼠成釉细胞内质网应激,并且通过内质网应激,引起细胞凋亡。

关键词: 氟, 成釉细胞, 内质网应激, 细胞凋亡

Abstract: PURPOSE: To study the effect of GRP-78 and caspase-12 on fluoride-induced endoplasmic reticulum stress and apoptosis in rat ameloblast, and explore whether fluoride-induced endoplasmic reticulum stress results in the occurrence of apoptosis. METHODS: The cell activity of ameloblast cultured in various concentrations of fluoride was measured by CCK8 and flow cytometry; Real-time RT-PCR and Western blot were used to analyze the endoplasmic reticulum chaperone GRP-78 and caspase-12 genes and the expression of related proteins. The data was analyzed with SPSS13.0 software package. RESULTS: With the increasing concentration of fluoride, the cell activity of rat ameloblasts decreased gradually, and flow cytometry also showed that the number of apoptosis gradually increased; Real-time RT-PCR and Western blot showed the expression of GRP-78 and caspase-12 increased while the fluoride concentration increased. CONCLUSIONS: Excessive fluoride induces endoplasmic reticulum stress of rat ameloblast, and leads to cell apoptosis.

Key words: Fluoride, Ameloblast, Endoplasmic reticulum stress, Apoptosis

中图分类号:

R780.1

李健,张颖,马林,张凯强,雷双,顾何锋. 氟对大鼠成釉细胞GRP-78和caspase-12表达的影响[J]. 上海口腔医学, 2015, 24(1): 1-5.

LI Jian,ZHANG Ying,MA Lin,ZHANG Kai-qiang,LEI Shuang,GU He-feng. The effect of fluoride on the expression of GRP-78 and caspase-12 in rat ameloblast[J]. Shanghai Journal of Stomatology, 2015, 24(1): 1-5.

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氟对大鼠成釉细胞GRP-78和caspase-12表达的影响

The effect of fluoride on the expression of GRP-78 and caspase-12 in rat ameloblast

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