CytC在原发性干燥综合征患者唇腺中的异常表达

上海口腔医学 ›› 2013, Vol. 22 ›› Issue (6): 680-683.

CytC在原发性干燥综合征患者唇腺中的异常表达

康媛媛，张英

(中国医科大学口腔医学院综合急诊科，辽宁省口腔医学研究所口腔黏膜病学研究室，辽宁沈阳 110002)

收稿日期:2013-04-19 修回日期:2013-06-10 出版日期:2013-04-12 发布日期:2013-04-12
通讯作者: 康媛媛，E-mail:kyandly@sina.com
作者简介:康媛媛(1982-)，女，硕士，讲师，主治医师
基金资助:
辽宁省科学技术计划项目(2011225020)；2013年院级青年科研启动基金(K101593-13-39)

Abnormal expression of CytC in the labial gland of patients with primary Sj?gren’s syndrome

KANG Yuan-yuan, ZHANG Ying

Department of Comprehensive Emergency, School of Stomatology, China Medical University, Liaoning Institute of Dental Research, Laboratory of Oral Mucosal Disease. Shenyang 110002, Liaoning Province, China

Received:2013-04-19 Revised:2013-06-10 Online:2013-04-12 Published:2013-04-12
Supported by:
Supported by Science and Technology Research Project of Liaoning Province (2011225020) and College Scientific Research Foundation for Youth in 2013 (K101593-13-39).

摘要/Abstract

摘要： 目的：研究原发性干燥综合征患者唇腺中细胞色素C表达水平的改变。方法：选择原发性干燥综合征患者35例和健康对照15例，采用免疫组化法和反转录-聚合酶链式反应(RT-PCR)检测2组唇腺组织中CytC蛋白和mRNA的表达，测量吸光度值，采用SPSS14.0软件包对结果进行Mann-Whitney检验，并与临床检验结果进行Spearman相关分析。结果：原发性干燥综合征患者唇腺组织中CytC在蛋白水平和mRNA水平均高表达，与健康对照组相比显著升高(P<0.05)，并与疾病的病程呈正相关。随着唇腺淋巴细胞浸润灶等级的增加，CytC的表达也随之升高(P<0.05)，但与其他临床特征之间不存在明显相关性。结论：CytC在原发性干燥综合征患者唇腺组织中表达增强，且与病理分级和疾病的病程相关。

关键词: 干燥综合征, 唇腺, 凋亡, 细胞色素C

Abstract: PURPOSE: To explore the expression of CytC in the labial glands of patients with primary Sj?gren’s syndrome(pSS). METHODS: Thirty-five cases with pSS and 15 controls were collected. Immunohistochemistry and RT-PCR were used to examine CytC protein and mRNA in labial glands of 2 groups. The integral optical density were measured and the data was analyzed by Mann-Whitney test with SPSS14.0 software package. The correlation between the clinical manifestations and the expression of CytC were calculated by Spearman’s analysis. RESULTS: The expression of CytC protein and mRNA in pSS were positively correlated with the disease course and significantly higher than those in control group (P<0.05). There was a positive correlation between the lymphocyte focus score and the expression of CytC, but no correlation with the other clinical parameters. CONCLUSIONS: The expression of CytC in pSS is increased,which is related to the disease course and the lymphocyte focus score.

Key words: Sj?gren’s syndrome, Labial gland, Apoptosis, CytC

中图分类号:

R781.7

康媛媛, 张英. CytC在原发性干燥综合征患者唇腺中的异常表达[J]. 上海口腔医学, 2013, 22(6): 680-683.

KANG Yuan-yuan, ZHANG Ying. Abnormal expression of CytC in the labial gland of patients with primary Sj?gren’s syndrome[J]. Shanghai Journal of Stomatology, 2013, 22(6): 680-683.

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