血管内皮细胞对牙周组织细胞增殖的影响

上海口腔医学 ›› 2013, Vol. 22 ›› Issue (5): 518-522.

血管内皮细胞对牙周组织细胞增殖的影响

丁进，尹元正

(上海交通大学医学院附属第九人民医院?口腔医学院牙周病科，上海市口腔医学重点实验室，上海 200011)

收稿日期:2013-03-26 修回日期:2013-04-12 出版日期:2013-10-10 发布日期:2013-10-10
通讯作者: 尹元正，Tel: 021-23271699-5282，E-mail:yinyuanzheng@gmail.com
作者简介:丁进(1987-)，男，硕士，住院医师，E-mail:cqxiaoding2009@126.com

The effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts

DIN Jin, YIN Yuan-zheng

Department of Periodontics, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2013-03-26 Revised:2013-04-12 Online:2013-10-10 Published:2013-10-10

摘要/Abstract

摘要： 目的：通过血管内皮细胞与牙周组织细胞复合培养模拟牙周组织再生环境，观察血管内皮细胞对牙周组织细胞增殖的作用规律，探讨血管内皮细胞对牙周组织修复再生的影响。方法：应用原代培养的血管内皮细胞，在Transwell嵌套中实现与人牙周膜成纤维细胞、牙龈成纤维细胞间的复合培养，分别于复合培养第2、4、6、8和10天，以细胞计数手段检测血管内皮细胞共存条件下2种牙周组织细胞的增殖情况，并与单独培养的2种牙周组织细胞作为对照，采用SPSS13.0软件包对数据进行统计学分析。结果：在血管内皮细胞存在条件下，2种牙周组织细胞的增殖速率均显著高于单独培养条件。血管内皮细胞存在时，牙周膜成纤维细胞增殖速率逐渐超越牙龈成纤维细胞(第6天起)，并在第8天细胞数量上超过牙龈成纤维细胞，差异具有显著性(P<0.01)。结论：血管内皮细胞的存在，对牙周组织细胞的增殖具有促进作用，对牙周膜成纤维细胞增殖的促进作用显著高于牙龈成纤维细胞。

关键词: 牙周再生, 血管内皮细胞, 细胞增殖

Abstract: PURPOSE: To investigate the effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts through a model mimicking the periodontal wound healing and regeneration. METHODS: Human vascular endothelial cells, periodontal ligament cells and gingival fibroblasts were co-cultured using a Transwell system. The number of each type of cells at 2, 4, 6, 8 and 10 days after co-culture were counted using a cell counter machine, while periodontal ligament cells and gingival fibroblasts single culture were used as control. The results were statistically processed with SPSS 13.0 software package. RESULTS: When vascular endothelial cell was present, the proliferation of periodontal ligament cells and gingival fibroblasts were faster than each single culture, the difference between them was significant (P<0.01). The proliferation of periodontal ligament cells was faster than gingival fibroblast as they were co-cultured with vascular endothelial cells(which happened at day 6), although the results were contrary when they were single cultured, the difference between them was significant (P<0.01). CONCLUSIONS: The presence of vascular endothelial cells can promote the proliferation of periodontal cells, and the effect is more prominent on human periodontal ligament cells than on gingival fibroblasts.

Key words: Periodontal regeneration, Vascular endothelial cells, Cell proliferation

中图分类号:

R781.4

丁进, 尹元正. 血管内皮细胞对牙周组织细胞增殖的影响[J]. 上海口腔医学, 2013, 22(5): 518-522.

DIN Jin, YIN Yuan-zheng. The effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts[J]. Shanghai Journal of Stomatology, 2013, 22(5): 518-522.

参考文献

[1] Melcher AH. On the repair potential of periodontal tissues[J]. J Periodontol, 1976, 47(5): 256-260.
[2] Gottlow J, Nyman S, Lindhe J, et al. New attachment formation in the human periodontium by guided tissue regeneration. Case reports[J]. J Clin Periodontol, 1986, 13(6): 604-616.
[3] Izumi Y, Aoki A, Yamada Y, et al. Current and future periodontal tissue engineering[J]. Periodontol 2000, 2011, 56(1): 166-187.
[4] Aspriello SD, Zizzi A, Spazzafumo L, et al. Effects of enamel matrix derivative on vascular endothelial growth factor expression and microvessel density in gingival tissues of periodontal pocket: a comparative study[J]. J Periodontol, 2011, 82(4): 606-612.
[5] Bertl K, An N, Bruckmann C, et al. Effect of enamel matrix derivative on proliferation/viability, migration, and expression of angiogenic factor and adhesion molecules in endothelial cells in vitro[J]. J Periodontol, 2009, 80(10): 1622-1630.
[6] Artese L, Piattelli A, de Gouveia Cardoso LA, et al. Immunoexpression of angiogenesis, nitric oxide synthase, and proliferation markers in gingival samples of patients with aggressive and chronic periodontitis[J]. J Periodontol,2010,81(5): 718-726.
[7] Sato E, Hashimoto H, Kamoi H, et al. Proliferation and tube formation of periodontal endothelial cells[J]. Hum Cell,2005,18(2): 83-91.
[8] DeCarlo AA, Cohen JA, Aguado A, et al. Isolation and characterization of human gingival microvascular endothelial cells[J]. J Periodontal Res, 2008, 43(2): 246-254.
[9] Kauvar AS, Thoma DS, Carnes DL, et al. In vivo angiogenic activity of enamel matrix derivative[J]. J Periodontol, 2010, 81(8): 1196-1201.
[10] Marx RE, Carlson ER, Eichstaedt RM, et al. Platelet-rich plasma: growth factor enhancement for bone grafts[J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 1998, 85(6): 638-646.
[11] Bertrand-Duchesne MP, Grenier D, Gagnon G. Epidermal growth factor released from platelet-rich plasma promotes endothelial cell proliferation in vitro[J]. J Periodontal Res,2010,45(1):87-93.
[12] Berthod F, Germain L, Tremblay N, et al. Extracellular matrix deposition by fibroblasts is necessary to promote capillary-like tube formation in vitro[J]. J Cell Physiol, 2006, 207(2): 491-498.
[13] Schlueter SR, Carnes DL, Cochran DL. In vitro effects of enamel matrix derivative on microvascular cells[J]. J Periodontol, 2007, 78(1): 141-151.
[14] Nagai N, Hirakawa A, Otani N, et al. Development of tissue-engineered human periodontal ligament constructs with intrinsic angiogenic potential[J]. Cells Tissues Organs,2009,190(6):303-312.
[15] 尹元正, 沈弛晶, 王丽珍, 等. 一种牙周细胞体外创面模型的构建[J]. 牙体牙髓牙周病学杂志, 2007, 17(9): 499-502.
[16] Nanci A, Bosshardt DD. Structure of periodontal tissues in health and disease[J]. Periodontol 2000, 2006, 40: 11-28.
[17] Ishikawa I, Iwata T, Washio K, et al. Cell sheet engineering and other novel cell-based approaches to periodontal regeneration[J]. Periodontol 2000, 2009, 51: 220-238.