氟对大鼠切牙成釉细胞内质网伴侣分子表达的影响

上海口腔医学 ›› 2013, Vol. 22 ›› Issue (5): 481-486.

• 基础研究 • 下一篇

氟对大鼠切牙成釉细胞内质网伴侣分子表达的影响

张凯强，张颖，刘璐，顾何锋，马林

(中国医科大学口腔医学院口腔预防科，辽宁省口腔医学研究所口腔预防教研室，辽宁沈阳 110002)

收稿日期:2013-02-14 修回日期:2013-04-10 出版日期:2013-10-10 发布日期:2013-10-10
通讯作者: 张颖，Tel：024-22895293，E-mail：zhangyingcmu@126.com
作者简介:张凯强(1987-)，男，在读硕士研究生，E-mail：zhangkaiqiang1208@gmail.com
基金资助:
国家自然科学基金(81072245)；辽宁省自然科学基金(20102278)

Effect of fluoride on the expression of endoplasmic reticulum chaperone in ameloblast of rat incisor

ZHANG Kai-qiang, ZHANG Ying, LIU Lu, GU He-feng, MA Lin

Department of Preventive Dentistry, School of Stomatology, China Medical University. Shenyang 110002, Liaoning Province, China

Received:2013-02-14 Revised:2013-04-10 Online:2013-10-10 Published:2013-10-10
Supported by:
Supported by National Natural Science Foundation of China (81072245) and Natural Science Foundation of Liaoning Province (20102278).

摘要/Abstract

摘要： 目的：研究不同浓度氟化物对大鼠切牙成釉细胞的影响，探讨内质网应激在氟斑牙形成中的作用。方法：30只Wistar大鼠随机分为3组，建立氟斑牙动物模型。观察大鼠切牙成釉细胞的形态学和GRP78、XBP-1、CRT和caspase-12表达的改变，采用MetaMorph显微图像分析软件和SPSS 13.0软件包对数据进行统计学分析。结果：随着饮水氟离子浓度的升高，切牙逐渐出现氟斑牙症状。免疫组化结果显示，CRT(F=11.72，P<0.05)、GRP78(F=27.42，P<0.05)、XBP-1(F=139.7，P<0.05)、caspase-12(F=43.91，P<0.05)表达随氟离子浓度的升高而升高,且各组间均具有显著性差异。结论：成釉细胞在一定浓度的氟化物作用下，其CRT、GRP78、XBP-1和caspase-12 均过表达，表明成釉细胞处于内质网应激状态，且caspase-12是导致细胞凋亡的重要途径。

关键词: 氟, 成釉细胞, 内质网应激

Abstract: PURPOSE: To investigate the effect of different concentrations of fluoride on the expression of endoplasmic reticulum chaperone, and to explore the mechanism of dental fluorosis in rat. METHODS: Thirty Wistar rats were randomly divided into 3 groups. Immunohistochemistry was used to detect the expression of CRT, GRP78, XBP-1 and caspase-12 in rat incisors. Metamorph microscope images analysis system and SPSS 13.0 software package was used to analyze the data. RESULTS: Typical features of dental fluorosis were found in the fluoride group. Results of immunohistochemistry showed that CRT (F=238.6, P<0.05), GRP78 (F=27.42, P<0.05), XBP-1 (F=139.7, P<0.05) and caspase-12 (F=43.91, P<0.05) were significantly different among the 3 groups. CONCLUSIONS: Excessive fluoride can increase the secretion of CRT, GRP78, XBP-1 and caspase-12 suggest the ameloblasts and in status of endoplasmic reticulum stress and caspase-12 plays an important role during ameloblast apoptosis.

Key words: Fluoride, Ameloblast, Endoplasmic reticulum stress

中图分类号:

R780.1

张凯强, 张颖, 刘璐, 顾何锋, 马林. 氟对大鼠切牙成釉细胞内质网伴侣分子表达的影响[J]. 上海口腔医学, 2013, 22(5): 481-486.

ZHANG Kai-qiang, ZHANG Ying, LIU Lu, GU He-feng, MA Lin. Effect of fluoride on the expression of endoplasmic reticulum chaperone in ameloblast of rat incisor[J]. Shanghai Journal of Stomatology, 2013, 22(5): 481-486.

参考文献

[1] Shore RC, Robinson C, Kirkham J, et al. An immunohistochemical study of the effects of fluoride on enamel development in the rat incisor[J]. Arch Oral Biol, 1993, 38(7): 607-610.
[2] DenBesten PK. Biological mechanisms of dental fluorosis relevant to the use of fluoride supplements[J]. Community Dent Oral Epidemiol, 1999, 27(1): 41-47.
[3] Zhang Y, Berger SA. Increased calcium influx and ribosomal content correlate with resistance to endoplasmic reticulum stress-induced cell death in mutant leukemia cell lines[J]. J Biol Chem, 2004, 279(8): 6507-6516.
[4] Liu H, Bowes RC 3rd, van de Water B, et al. Endoplasmic reticulum chaperones GRP78 and calreticulin prevent oxidative stress, Ca2+ disturbances, and cell death in renal epithelial cells[J]. J Biol Chem, 1997, 272(35): 21751-21759.
[5] Sharma R, Tsuchiya M, Bartlett JD, et al. Fluoride induces endoplasmic reticulum stress and inhibits protein synthesis and secretion[J]. Environ Health Perspect, 2008, 116(9): 1142-1146.
[6] DenBesten PK, Yan Y, Featherstone JD, et al. Effects of fluoride on rat dental enamel matrix proteinases[J]. Arch Oral Biol, 2002, 47(11): 763-770.
[7] Dihaz H, Asif AR, Agarwal NK, et al. Proteomic analysis of cellular response to osmotic stress in thick ascending limb of Henle’s loop (TALH) cells[J]. Mol Cell Proteomics, 2005, 4(10): 1445-1458.
[8] Hammadi M, Oulidi A, Gackière F, et al. Modulation of ER stress and apoptosis by endoplasmic reticulum calcium leak via translocon during unfolded protein response: involvement of GRP78[J]. FASEB J, 2013, 27(4):1600-1609.
[9] Lee AS. The ER chaperone and signaling regulator GRP78/BiP as a monitor of endoplasmic reticulum stress[J]. Methods, 2005, 35(4): 373-381.
[10] Rasheva VI, Domingos PM. Cellular responses to endoplasmic reticulum stress and apoptosis[J]. Apoptosis, 2009, 14(8): 996-1007.
[11] Kubota K, Lee DH, Tsuchiya M, et al. Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation[J]. J Bilo Chem, 2005, 280(24): 23194-23202.
[12] 张雪莉, 席淑华, 郭晓英, 等. 氟对大鼠切牙成釉细胞形态影响及褪黑素拮抗氟作用研究[J].中国实用口腔科杂志, 2011, 4(3): 159-161.
[13] Fujimoto T, Yoshimatsu K, Watanabe K, et al. Overexperssion of human X-box binding protein 1 (XBP-1) in colorectal adenomas and adenocarcinomas[J]. Anticancer Res, 2007, 27(1A): 127-131.
[14] Van Schadewijk A, van’t wout EF, Stolk J, et al. A quantitative method for detection of spliced X-box binding protein-1(XBP1) mRNA as a measure of endoplasmic reticulum (ER) stress[J]. Cell stress Chaperones, 2012, 17(2): 275-279.
[15] Sriburi R, Bommiasamy H, Buldak GL, et al. Coordinate regulation of phospholipid biosynehesis and secretory pathway gene expression in XBP1 induced endoplasmic reticulum biogenesis[J]. J Biol Chem, 2007, 282(10): 7024-7034.
[16] Honda A, Abe S, Hiroki E, et al. Activation of caspase 3, 9, 12, and Bax in masseter muscle of mdx mice during necrosis[J]. J Muscle Res Cell Motil, 2007, 28(4-5): 243-247.
[17] Nakagawa T, Zhu H, Morishima N, et al. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta[J]. Nature, 2000, 403(6765): 98-103.

氟对大鼠切牙成釉细胞内质网伴侣分子表达的影响

Effect of fluoride on the expression of endoplasmic reticulum chaperone in ameloblast of rat incisor

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	郁莹, 翁清清, 罗银月, 姚姝冉, 易芳羽, 张颖. 过量氟对小鼠髁突软骨损伤及自噬相关因子LC3、p62表达的影响[J]. 上海口腔医学, 2024, 33(2): 113-116.
[2]	裴婧, 张林, 贾云香, 陈蔚华. BRAF V600E在成釉细胞瘤、成釉细胞癌和囊肿中的表达[J]. 上海口腔医学, 2023, 32(6): 630-634.
[3]	王雪纯, 吴艳棋, 梅鹏, 张博钧, 朱敏. 低强度牵张应力对异丙肾上腺素诱导下牙周膜成纤维细胞炎症反应的影响[J]. 上海口腔医学, 2022, 31(3): 225-231.
[4]	罗号, 袁壮, 吴开柳, 贺捷, 孟箭. 92例上颌骨成釉细胞瘤不同手术方式的效果评价[J]. 上海口腔医学, 2022, 31(1): 71-74.
[5]	邵云, 程庆涛, 何欢, 李晶, 朱微燕, 刘嘉靓. 2种不同舒适化技术在60例高血压患者阻生牙拔除术中的应用评价[J]. 上海口腔医学, 2022, 31(1): 109-112.
[6]	虞瑾, 曾晓莉, 江一巍, 王沪宁, 张皓, 笪东欣, 张颖. 应用决策树模型评价3~5岁儿童含氟涂料防龋的经济学效果[J]. 上海口腔医学, 2021, 30(6): 639-643.
[7]	吴慧, 刘桦, 刘文, 刘义琮, 杨芳. 玻璃离子保护膜与氟保护漆对牙釉质脱矿的预防和再矿化作用[J]. 上海口腔医学, 2021, 30(5): 493-497.
[8]	汤颖, 沈忆芬, 刘超, 邱吟枫, 顾永春, 于金华. 高浓度氟对人牙周膜干细胞凋亡的影响[J]. 上海口腔医学, 2021, 30(4): 360-366.
[9]	邵云, 盛璐, 何欢, 刘慧, 陆萌萌, 祁铭. 右美托咪定结合氟比洛芬酯在局麻下多颗复杂牙拔除术中的应用效果评价[J]. 上海口腔医学, 2021, 30(3): 302-305.
[10]	孙旸, 高承志. 含氟涂漆与CPP-ACP或生物活性玻璃对根龋的作用评价[J]. 上海口腔医学, 2020, 29(1): 46-50.
[11]	张爱华, 李婧. 氟化钠护齿剂与窝沟封闭剂用于防治学龄前儿童龋齿的效果评价[J]. 上海口腔医学, 2019, 28(5): 553-556.
[12]	林静, 姚华. 窝沟封闭术联合氟保护漆在预防5~8岁儿童龋齿中的效果评价[J]. 上海口腔医学, 2019, 28(4): 384-387.
[13]	嵇国平, 田一宏, 刘美希, 沈莹, 沈刚. 内质网应激关键因子PREK在应力加载下成肌细胞凋亡中的作用及机制[J]. 上海口腔医学, 2019, 28(3): 259-263.
[14]	苏红如, 杨忍忍, 钱文昊, 余金明. 氟化钠护齿剂在预防学龄前儿童乳牙龋齿中的疗效观察[J]. 上海口腔医学, 2019, 28(1): 48-42.
[15]	汪嘉，何智妍，冉淑君，朱来宽. 耐氟变形链球菌对树脂-牙本质粘接强度的影响[J]. 上海口腔医学, 2018, 27(3): 239-243.